Immunohistochemical expression of p53, ki-67, tenascin, and fibronectin in giant cell fibroma and traumatic fibroma of the oral mucosa

Ingrid Gomes de Oliveira; Adriana Aparecida Silva da Costa; Daniela Pereira Meirelles; Thalita Soares Tavares; João de Jesus Viana Pinheiro; Ricardo Alves de Mesquita; Martinho Campolina Rebello Horta; Patrícia Carlos Caldeira

doi:10.5327/2525-5711.263

Authors

Ingrid Gomes de Oliveira Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0009-0009-6958-5952
Adriana Aparecida Silva da Costa Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0000-0001-7655-8666
Daniela Pereira Meirelles Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0000-0002-4698-3992
Thalita Soares Tavares Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0000-0001-7220-1360
João de Jesus Viana Pinheiro Universidade Federal do Pará, Center for Biological and Health Sciences – Belém (PA), Brazil. https://orcid.org/0000-0002-6914-6441
Ricardo Alves de Mesquita Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0000-0003-3207-4007
Martinho Campolina Rebello Horta Pontifícia Universidade Católica de Minas Gerais, Institute of Biological and Health Sciences, Department of Dentistry, Belo Horizonte (MG), Brazil. https://orcid.org/0000-0003-0192-5614
Patrícia Carlos Caldeira Universidade Federal de Minas Gerais, School of Dentistry, Oral Pathology and Surgery – Belo Horizonte (MG), Brazil. https://orcid.org/0000-0002-9179-0145

DOI:

https://doi.org/10.5327/2525-5711.263

Keywords:

Giant cell fibroma, immunohistochemistry, Ki-67, P53, Tenascin

Abstract

Objective: This study aimed to compare the immunoexpression of p53, ki-67, tenascin, and fibronectin between giant cell fibroma (GCF) and traumatic fibroma (TF), in order to explore a benign neoplastic or a reactive nature of GCF.

Methods: A cross-sectional study was conducted. Samples of GCF and TF were retrieved from the files of Oral Pathology Service, matched by site and size. Immunohistochemistry for p53, ki-67, tenascin, and fibronectin was evaluated in the superficial and deep regions of the lesions using the Image J Software. The number of positive cells was determined for p53 and ki-67, and the positive area was established for tenascin and fibronectin. Statistical analysis was performed with Mann-Whitney and independent t-tests (p≤0.05).

Results: Comparing to TF, GCF showed higher expression of p53 protein in superficial (p=0.009) and deep regions (p=0.027), as well as higher tenascin expression in deep regions (p=0.000). Ki-67 and fibronectin immunoexpression did not differ between GCF and TF (p>0.05).

Conclusions: The results of the present study seem supportive of a benign neoplastic nature of GCF, rather than a reactive one, especially considering the p53 and tenascin expression. Further studies with larger samples and broader markers should confirm this hypothesis.

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Immunohistochemical expression of p53, ki-67, tenascin, and fibronectin in giant cell fibroma and traumatic fibroma of the oral mucosa

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